Calcium Dependent ATPase in Mast Cell Membranes and Its Role in Histamine Release
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An assay was developed to detect minute amounts of phosphate split from ATP by ion stimulated ATPases. Erythrocyte ghosts were used as a known source of both Ca++ and Na+-K+ ATPases to show the effectiveness of the assay. Mast cells were then assayed for the presence of these enzymes, with negative results for both. There was some evidence that Ca++ ATPases inhibitors inhibited histamine release, but the specificity and mechanism of the inhibition has not been shown.