TLC Applied in the Bio-Assay of Neuroinhibitor Substances Present in Mammalian Brain Extracts
The inhibitory activity of Factor I has been attributed entirely to GABA by several workers. Recent studies report that GABA does not account for the total amount of inhibition present in Factor I. L-Glutamic acid has been reported to inhibit acetylcholine induced contractions of the crayfish hindgut (3.4xlO-4 M). This was about 20 times the concentration of GA8A reported to be needed to produce the same result. The purpose of this experiment was to determine the active portion of the extract, specifically if it was GA8A or L-Glutamic acid, and what portion of the inhibition could be attributed to each.