The Isolation and Characterization of Escherichia Coli Methyl-Deficient Isoaccepting Transfer Ribonucleic Acids
Nickoloff, Brian Jeffrey
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The focus of this research has been the application of the reversed-phase system (RPC-5) as an analytical tool for the rapid isolation and identification of isoaccepting formylating species or methionine tRNA. Also the purified methyl-deficient species of tRNA could be used in studies of the function of individual nucleotides in tRNA, especially those present in low concentrations, to help explain the high specific recognition of RNAs by their cognate aminoacyl-tRNA synthetases. Finally, the enzymatic modification of the four major basis in tRNA have significant effects on the function of tRNA in protein synthesis. The aminoacylation activity of our methy1-deficient species is compared to the normal. fully methylated tRNA, and the nucleoside content or these tRNAs have been analyzed and the results interpreted in relation to the aminoacylation activity as well as methyl-group acceptances of these isoaccepting species.