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dc.contributor.advisorWuest, Simone
dc.contributor.advisorBielekova, Bibiana
dc.contributor.authorPandit, Roopa S.
dc.date.accessioned2011-01-10T14:21:37Z
dc.date.available2011-01-10T14:21:37Z
dc.date.issued2010
dc.identifier.urihttp://hdl.handle.net/10920/19649
dc.descriptionvi, 26 p.en_US
dc.description.abstractMitochondria and oxidative stress are hypothesized to be involved in the pathogenesis of primary progressive (PP-MS) and secondary progressive multiple sclerosis (SP-MS). Lactate and transferrin are believed to be biomarkers that could be measured in the cerebrospinal fluid (CSF) of MS patients. These biomarkers could potentially be involved in PP-MS and SP-MS. Conditions were established for measuring lactate and transferrin levels in CSF using the Biovision Lactate Assay Kit and Genway Transferrin ELISA Kit. Using these kits, the concentration of CSF that was necessary to fall within the respective standard curves for lactate (1:10 in buffer) and transferrin (1:300 in buffer) was concluded. A baseline study was completed using cerebrospinal fluid from 31 patient donors through lumbar puncture to determine variances in lactate and transferrin levels in clinically diagnosed non-MS, relapsing-remitting MS, and primary-progressive MS/secondary-progressive MS patients. The effect of Zenapax (Daclizumab) on oxidative stress was also assessed using a cohort of 14 patients before and after 6 months of treatment using the established biomarkers. However, there was no clear trend between lactate or transferrin concentration and clinical diagnosis of multiple sclerosis. Zenapax had no clear effect on oxidative stress biomarkers as some patients increased and some decreased in lactate and transferrin levels. Future studies using the reproducible and precise protocols are needed to determine the effects of antioxidants and other drugs.en_US
dc.description.sponsorshipNational Institute of Neurological Disorders and Stroke. National Institutes of Health. Bethesda, Maryland.
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.publisherKalamazoo Collegeen_US
dc.relation.ispartofKalamazoo College Chemistry Senior Individualized Projects Collection
dc.relation.ispartofseriesSenior Individualized Projects. Chemistry.;
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder. All rights reserved.
dc.titleBiomarkers of Oxidative Stress in the Cerebrospinal Fluid of Multiple Sclerosis Patientsen_US
dc.typeThesisen_US


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  • Chemistry Senior Individualized Projects [860]
    This collection includes Senior Individualized Projects (SIP's) completed in the Chemistry Department. Abstracts are generally available to the public, but PDF files are available only to current Kalamazoo College students, faculty, and staff.

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