Biomarkers of Oxidative Stress in the Cerebrospinal Fluid of Multiple Sclerosis Patients
Pandit, Roopa S.
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Mitochondria and oxidative stress are hypothesized to be involved in the pathogenesis of primary progressive (PP-MS) and secondary progressive multiple sclerosis (SP-MS). Lactate and transferrin are believed to be biomarkers that could be measured in the cerebrospinal fluid (CSF) of MS patients. These biomarkers could potentially be involved in PP-MS and SP-MS. Conditions were established for measuring lactate and transferrin levels in CSF using the Biovision Lactate Assay Kit and Genway Transferrin ELISA Kit. Using these kits, the concentration of CSF that was necessary to fall within the respective standard curves for lactate (1:10 in buffer) and transferrin (1:300 in buffer) was concluded. A baseline study was completed using cerebrospinal fluid from 31 patient donors through lumbar puncture to determine variances in lactate and transferrin levels in clinically diagnosed non-MS, relapsing-remitting MS, and primary-progressive MS/secondary-progressive MS patients. The effect of Zenapax (Daclizumab) on oxidative stress was also assessed using a cohort of 14 patients before and after 6 months of treatment using the established biomarkers. However, there was no clear trend between lactate or transferrin concentration and clinical diagnosis of multiple sclerosis. Zenapax had no clear effect on oxidative stress biomarkers as some patients increased and some decreased in lactate and transferrin levels. Future studies using the reproducible and precise protocols are needed to determine the effects of antioxidants and other drugs.