Detection of preconcentrated mRNA through a molecular beacon assay for the early detection of breast cancer metastasis
Among all cancers, breast cancer has the second highest incidence rate among women. However, early detection of the disease has led to increased treatment options for patients. To obtain accurate diagnoses, biomarkers for breast cancer can be monitored and the accuracy is even further increased when two or more biomarkers are monitored. To detect multiple biomarkers in a sample, molecular beacons (MB) can be used due to their high specificity and sensitivity. Two molecular beacons were designed specific to mRNA transcripts of two different breast cancer biomarkers for simultaneous detection in a single sample. Each MB uses a different fluorophore-quencher pair; therefore the fluorescence output caused by the target-mRNA-MB interaction can be monitored for each biomarker simultaneously. The total time for the designed assay is significantly shorter than current methods based on RT-PCR. The limit of detection for each biomarker has been reduced with pre-concentration using beads and a capture oligonucleotide sequence complementary to the monitored biomarkers.
Showing items related by title, author, creator and subject.
Spiral Plating Technique Applied to the Detection of Antagonism Between Two Oral Species of Black-Pigmented Bacteroides Wilson, Ann C. (1983)Various strains of black-pigmented Bacteroides were screened for antagonistic interactions by using a spiral plating technique. The initial screening procedure included cross combinations of Bacteroides gingivalis, Bacteroides ...
Comparison of Tissue Digestion Techniques for Detecting Toxoplasma gondii in Cockles (Anadora granosa) along the Coast of Thailand Kanjanakuha, Patchamol (Kalamazoo, Mich. : Kalamazoo College, 2014)Toxoplasma gondii is a parasite in the phylum Apicomplexa that causes an infection called toxoplasmosis in a majority of endothermic vertebrates. It is believed that felids, such as domestic cats, are the only definitive ...
Detection of Toxicity with an Application of a Phosphorescent Oxygen Probe, Luxcel A65N, for an Assay System Screening Cellular Respiration Stallings, Sara (Kalamazoo, Mich. : Kalamazoo College., 2005-04-29)Due to the changing paradigm of drug testing, from in vivo to in vitro testing, new methods and assays are being investigated and developed to make more accurate and sensitive assays. When developing new assays, an important ...