Effect of the Ototoxins Neomycin and Ouabain on the Spiral Ganglion Neuron and Hair Cells of the Mouse Cochlea
Weir, William B.
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Within society, hearing loss is a devastating problem as once they are damaged, cochlear hair and neural cells are permanently gone. Regeneration of these vital cells is a focus of stem cell research in otolaryngology. To determine a better model of test animal deafening, the ototoxin ouabain and the aminoglycoside neomycin were injected into the mouse cochlea via the round window. This served to evaluate the viability of selectively damaging the hair cells, spiral ganglion neuron, or the central process of the cochlea. Four days post-deafening, ABR measurements indicated significant hearing loss confirming success of deafening. One week post-deafening, ABR measurement indicated further hearing loss progression. Ouabain had selectively targeted the spiral ganglion neuron with little to no damage of the cochlear hair cells. Neomycin had almost completely damaged the hair cells with slight injury also occurring to the spiral ganglion. These findings indicate that with further research a completely efficient method for modeling ototoxic deafening in humans can be developed.