Effects of a Tethered Chromophore on the Dynamics of Phosphocholine Lipid Vesicle Systems
Miller, Kelly K.
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We have investigated the dynamics of unilamellar vesicles of phosphocholine lipids and lyso lipids tagged with a chromophore. The chromophore, NBD, is tethered to the acyl chain of the tagged lipids. Prior research has indicated that the presence of a polar chromophore on acyl chains may cause the non-polar tails to loop back toward the polar lipid headgroup moiety. This work seeks to investigate this phenomenon further using both phospholipids and lyso lipids. Using time-correlated single photon counting, fluorescence anisotropy decay data was collected and analyzed. Anisotropy decay curves were generated and fit with single exponential decay equations. A more stable local environment around the chromophore was found in a phospholipid vesicle than that of a lyso lipid micelle. Findings are supported by fluorescence lifetime and anisotropic reorientation data.