Selected Studies of Serum Alkaline Phosphatase Isoenzymes by Starch Gel Electrophoresis
Bernstein, Joel Richard
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The present study is concerned with the separation of the serum fractions of alkaline phosphatase in order to relate certain clinical disorders with their respective isoenzyme patterns. The clinical area of major interest to this study was that of gynecology. Special emphasis was put upon the investigation of endometrial carcinoma and associated disorders(as endometrial hyperplasia or abnormal uterine bleeding). It has been demonstrated by Atkinson and Gusbergll and Long and Doko that there is a decrease in tissue alkaline phosphatase associated with endometrial carcinoma. Another study, done on patients admitted for abnormal uterine bleeding, showed that the serum alkaline phosphatase is also low in endometrial carcinoma. This study was repeated to measure leukocyte alkaline phosphatase in patients with cancer of the endometrium and it showed a diminution to complete absence of the enzyme. Thus it can be seen that endometrial carcinoma appears to be associated with a decrease in the amount of alkaline phosphatase present in tissue, serum, and leukocytes.