Structural Studies of a High Molecular Weight Material Isolated During the Purification of the Protein Nisin
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Authors
Burling, Charles Kenneth
Issue Date
1972
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
This project was undertaken
in an attempt to do a structural determination of the high
molecular weight component. The question of whether the material
was actually high molecular weight material as well RS the structure
and form of molecular interaction served as the basis for the
study. Amino acid analysis of all nisin samples which underwent
column chromatography, redistribution by a counter current
distribution apparatus (upper phase butanol, lower phase 25%
acetic acid) and reactions with methyl mercaptain, cyanogen
bromide, or 1-fluoro-2,4-dinitrophenyl benzene were used in
an attempt to identify the material.
Description
111 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder. All rights reserved.