Preliminary Steps toward HPLC Simultaneous Determinations of Vitamin D3 and A Alcohol in Cod Liver Oil: An Essay on the Optimization of Seperation in Reverse-Phase High Performance Liquid Chromatography

Abstract

A number of separation techniques in high performance liquid chromatography have been developed over the past years which quantify the amount of vitamin D3 and A in various type of samples as individual or simultaneous determinations. A necessarily lengthy analysis time however is evident in these methods, particularly in dealing, with samples of cod liver oil. The aim of this project then is to develop a rapid and quantitative procedure by optimizing the best possible chromatographic system available to date. To achieve this goal, it is essential that the retention mechanism in the employed system be thoroughly understood so as to guide the practicing chromatographer into sensible undertakirgs. A review of the mechanism of the chosen system is consequently discussed at length in this paper.