Amino Acid Analysis of L5178Y Sensitive and Resistant Ascities Leukemia Cells after L-Asparaginaw Treatment Using High Performance Liquid Chromatography and Glycine Inhibition of L-Asparaginaw Effects
Smith, Rick J.
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It is hypothesized in the lab of Sheldon M. Schuster Ph.D that L-asparaginase inhibits the growth of leukemia tumor cells by inhibiting the pathway by which glycine is produced from asparagine and glyoxylate. Cells which are sensitive to L-asparaginase are such because they lack an alternative pathway of producing glycine after the pathway from glyoxylate and asparagine to glycine has been inhibited. Resistant cells, on the other hand, are able to survive because they have a means of producing glycine that does not involve asparagine. This pathway takes place in the mitochondria in which serine is converted into glycine by the enzyme serine-hydroxy-methyltransase. The overall goal of this research is to repeat the above experiment using ascites leukemia tumor cells where the only difference between resistant and sensitive cells is the fact that one group of tumor cells is resistant to L-asparaginase and the other group is sensitive.