The Terminal Amino Acids and the Affect of Perchloric Acid on the Urea Extractable and Histidine-Rich Epidermal Proteins in the Newborn Rat
Sibrack, Laurence Allan
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The task of this thesis is to report on structural ana1ysis of these two protein fractions and the affect of perchloric acid. This will be done by analyzing the amino- and carboxyl- terminal amino acids. The amino- terminal residue will be approached by the dinitrofluorobenzene technique of Sanger (1945) modified by Frankel-Conrat (1955). The carboX71- end group will be approached by the use or carboxypeptidase first used by Lens (1949) ane also reported by Frankel-Conrat (1955). This terminal amino acid determination should elucidate on the nature of the chemical bond cleaved due to the action of PCA.