The Kinetics of Antibody Fixation to Lymphocytes
Kooistra, Jon Brent
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The purpose of this research was to provide accurate measurements of the primary binding of both normal gamma globulin and ALS preparations. to viable lymphocytes. In addition, the kinetics of this antigen-antibody reaction were investigated. Using 125 I-labeled antibody preparations, association, dissociation and equilibrium constants of the reaction of antibody with lymphocytes were determined. In addition, an equilibrium dialysis technique was developed in order to provide a direct measurement of an association constant for the formation of the antigen-antibody complex.