Subcellular Fractionation of Thymocytes for Preparation of Specific Antisera
Lasswell, Lynn Alan
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The present study was undertaken in order to produce pure suboellular fractions from thymocytes. These fractions were used to inject rabbits in anticipation of eliciting more highly specific thymocyte antisera (ATS). By the use of this method we hoped to produce antisera with the following characteristics: 1) increased numbers of immunosuppressive molecules; 2) lowered toxicity of the antisera, as toxic antibodies have been shown to form in response to non-lymphoid contaminants of the stimulating antigen; 3) maintenance of immunosuppressive potency with continued immunization which can be maintained by reducing the development of non-immunosuppressive antibodies; 4) development of antibody which has selectivity for the thymus-derived lymphocyte, the cell type thought to be the site of action of ALS activity.