Effect of Temperature on the Cell Cycle of L1210 Leukemia Cells in Culture
Cooperrider, Peter Armitage
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The purpose of our study was to find means whereby we could synchronize L12l0 cells. High levels of thymidine (TdR) have been used to synchronize cells for the purpose of testing antibiotics that interfere only with the metabolism of a specific phase. High level of TdR has the following effects: 1) DNA synthesis is inhibited and the cells in S are prevented from proceeding to G2. 2) Cells in G2, M, and Gl progress through the cycle and are blocked at the beginning of S. 3) This is a reversible block, and cells proceed normally through the cycle when the TdR is removed.