A Study of the Level and Isoenzyme Pattern of Various Transaminases in the Chinese Hamster
Seaborne, Sharlyne B.
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The diabetic Chinese hamster has been used to study the respective level and isoenzyme pattern of serine dehydratase (SDH), tyrosine aminotransferase (TAT), aspartate aminotransferase. (AsAT), alanine aminotransferase (AlAT), and glutamate dehydrogenase (GDH) in the liver and heart tissues. The actual assays and electrophoretic work employed are based on methods developed in relation to studying these and other enzymes in the rat, pig, rabbit, dogfish, and sheep. The attempt to characterize these five enzymes in the Chinese hamster has helped to define further the effect of the disease diabetes mellitus on the gluconeogenesis process in the animal as well as man. A comparative analysis of the liver enzymatic activities, defined as units/mg protein and units/gm tissue, for these transaminases suggests the following trend in the diabetic as well as non-diabetic control Chinese hamster: AsAT > AlAT > SDH > TAT. The pattern of enzymatic activity in the heart is AsAT > AlAT.