An Assay to Determine the Level of Glyburide in Human Serum
Skinner, Gordon R.
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Glyburide Micronase, is an orally active sulfonylurea used in the control of maturity onset diabetes, for which FDA marketing approval is pending. A rapid, specific, and sensitive assay procedure capable of measuring intact glyburide in blood level amounts is desirable. This method would facilitate monitoring clinical blood levels and pharmacological studies. Because of the potency of the drug only a low therapeutic dosage is necessary (2.5-10 mg). The low dosage requires an analytical procedure sensitive to the lower nanogram/ml range. During the S.I.P. quarter, the author worked on such an assay under the direction of W. J. Adams, Ph.D., at The Upjohn Company, Kalamazoo, Michigan. Using a few extractions and high performance liquid chromatographic apparatus, the author could detect a lower limit of 4 ng glyburide/ml serum. This procedure is fairly fast, sensitive, specific and has the potential of clinical applications.