Effect of Rat Weight on Prostaglandin E1 Binding Sites in Adipocyte Plasma Membranes
Baldwin, Creighton W.
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A possible relationship between rat size or age and prostaglandin E1 receptor activity has been found. Rat adipocyte plasma membrane sacs from 50 gram male rats that were recently weaned bound no prostaglandin El. Binding activity was first observed with rats that weighed approximately 100 grams after being fed a normal rat chow diet for about a week. The prostaglandin El binding then increased in an almost linear fashion with increasing rat weight. By utilizing Lineweaver-Burk plots binding constants were also obtained, but there appeared to be no significant difference in the binding constant regardless of rat size or age. An attempt was made to obtain rats,) which would have abnormally low PGEl binding activity. This was done by feeding recently weaned rats a high fat diet. Preliminary results indicate that binding has been reduced presumably through an inhibition of the synthesis of the protein binding sites. However, the study is incomplete and deserves further investigation.