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dc.contributor.authorWood, Brianne M.
dc.description1 broadsideen_US
dc.description.abstractNeisseria gonorrhoeae, is a gram negative diplococcus, and is the causative agent for the sexually transmitted disease gonorrhea. Developments of a vaccine for gonococcal infection have also been unsuccessful because no cross-protective immunity can be attained. N. gonorrhoeae targets and attacks mucosal tissue via virulence factors: Type IV Pilus, Opacity-associated proteins (Opa), porA and porB porin channels, as well as high antigenic variation. The purpose of this study was to discover regulators within the Neisseria genome that could affect the expression of NG1684, thereby responding to adherence and facilitating invasion of host cells. Information about new regulators may be of clinical significance as it could lead to novel treatments for gonorrhea, as an alternative to the current use of antibiotics. By completing this heterologous screen we have found many genetic implications affecting NG1684 gene expression, the most up-regulated gene when gonococci adhere to human epithelial cells. It is possible that these genes encode products involved in pathogenesis. One known implication comes from the insert in sample pBW03, which encodes for the NnRs like proteins. Another implication could possibly arise from the plasmid insert of transformant pBW01 as it encodes for genes of the N. gonorrhoeae strain MS11 pathogenicity island. This island, separate from the sequence homology we discovered on the pBW01 plasmid insert, encodes for a Type IV secretion system, which in other pathogens serves a large role in pathogenesis.en_US
dc.description.sponsorshipKalamazoo College. Department of Biology. Diebold Symposium, 2010en_US
dc.publisherKalamazoo, Mich. : Kalamazoo Collegeen_US
dc.relation.ispartofKalamazoo College Diebold Symposium Presentation Collectionen
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.en
dc.titleA Heterologous Screen of the Neisseria gonorrhoeae Genetic Library and its Effects on the Expression of the Gene of Unknown Function, NG1684en_US

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  • Diebold Symposium Posters and Schedules [479]
    Poster and oral presentations by senior biology majors that include the results of their Senior Integrated Projects (SIPs) at the Diebold Symposium. Abstracts are generally available to the public, but PDF files are available only to current Kalamazoo College students, faculty, and staff.

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