In Vitro Methods for Measuring Incompatibility in the Canine : Lymphocyte Purification and the Mixed Leucocyte Culture Test
Rowlett, André Louis
MetadataShow full item record
The aim of the research was to obtain canine lymphocytes (LYM) in excess of 80% of the total leucocytes, while reducing the number of polymorphonucleocytes (PMN) to less than 20% of the total leucocytes in order to set up unidirectional mixed leucocyte culture (MLC) tests. When PMN are in greater quantity than this they influence MLC reactivity. A procedure involving phagocytosis of carbonyl iron particles by the PMN proved to be successful. The average yield of purified LYM from 20 cc of whole blood was 2.55 x 107 cells and the PMN comprised an average of 15.76% of this value. Initial progress of MLC tests in 50 cc conical bottom tubes and in microplate cultures is also reported.