In Vitro Methods for Measuring Incompatibility in the Canine : Lymphocyte Purification and the Mixed Leucocyte Culture Test
Loading...
Authors
Rowlett, André Louis
Issue Date
1974
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
The aim of the research was to obtain canine lymphocytes (LYM) in excess of 80% of the total leucocytes, while reducing the number of polymorphonucleocytes (PMN) to less than 20% of the total leucocytes in order to set up unidirectional mixed leucocyte culture (MLC) tests. When PMN are in greater quantity than this they influence MLC reactivity. A procedure involving phagocytosis of carbonyl iron particles by the PMN proved to be successful. The average yield of purified LYM from 20 cc of whole blood was 2.55 x 107 cells and the PMN comprised an average of 15.76% of this value. Initial progress of MLC tests in 50 cc conical bottom tubes and in microplate cultures is also reported.
Description
iii, 36 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder. All rights reserved.