The In Vitro Hydrolysis of 3-oxa-4, 5, 6-trinor-inter-m-phenylene-PGE1-amide
Platelet aggregation has been identified as a possible precipitating event in heart attack, stroke and related vascular disorders. A drug which inhibits platelet aggregation may be useful in the treatment and/or prevention of such diseases. Certain prostaglandin free acids and amides have been shown to be potent inhibitors of platelet aggregation. It has also been shown that PG-amides hydrolyze to the free acids in vitro and in vivo. An animal model which possesses hydrolytic capacity similar to the human would be useful in evaluating prostaglandin amides for their potential usefulness in humans. This project studied the hydrolytic capacity of plasma enzymes from humans, monkeys, guinea pigs and rats. 3-oxa- 4,5,6-trinor-inter-rn-phenylene-PGE1-amide was used to measure the in vitro hydrolytic capacity of these animal models.