Cultured Mouse Lens Epithelium: Methods of Cultivation and Characteristics of the Cultures
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The procedures for culturing Nakano and normal mouse lens epithelium were developed in the NEI laboratory by Dr. Y. Tsunematsu and Dr. Paul Russell. My goals were to grow both kinds of cells in culture and to record the morphological development of the cultures. I also hoped to examine two characteristics of the cultures for significant differences between Nakano and normal cells: the growth characteristics, and the glucose-6-phosphate dehydrogenase/hexokinase ratio, (an indication of the type of cellular metabolism).
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