Development of an Experimental Set-Up for the Separation of Proteins and Other Biolecules by Capillary Zone Electrophoresis
Goodwin, Jay Thomas
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Capillary zone electrophoresis (CZE) is a hybrid analytical technique incorporating the separation capabilities of high-performance liquid chromatography and electrophoresis. It is a monophasic separation technique, meaning that there is no partitioning of the analyte between separate phases. Separations occur on the basis of differences in migration velocities within an applied electric field. An on-column UV detector is utilized for detection of the migrating zones within the vitreous silica capillary. The effects of changes in various operational parameters upon the peak characteristics of certain analytes are examined. CZE is performed upon separate solutions of insulin and myoglobin in order to determine if separations of proteins are feasible with the present apparatus. An approximate value of the diffusion coefficient is determined for myoglobin.