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dc.contributor.authorMartell, Robert E.
dc.descriptionvi, 51 p.en_US
dc.description.abstractThe goal of this project was to characterize the binding site on protein kinase C (PKC) for tumor promoting phorbol esters. With this information, the hope is that an inert analog of the tumor promoting substances could be made and thus could halt the tumor promotion process. In previous research it has been shown that diacylglycerols (DAG) bind competitively with phorbol esters (PE) to PKC. Because of this and the similar effects that both DAG and PE elicit, it has been proposed that these two compounds are endogenous analogs. The thrust of this project is to obtain direct proof of this by gaining physical evidence that both bind to the same site. This is done using spin and fluorescent labels to look at binding immobilization. This project involves preparing fluorescently and spin labeled DAG’s and a fluorescently labeled PE. The binding affinity of the DAG’s are then characterized and the binding immobilization studies are done. For this project, I prepared and purified the DAG’s and characterized their binding to PKC.en_US
dc.description.abstractIf you are not a current K College student, faculty, or staff member, email to request access to this SIP.
dc.publisherKalamazoo Collegeen_US
dc.relation.ispartofKalamazoo College Chemistry Senior Individualized Projects Collection
dc.relation.ispartofseriesSenior Individualized Projects. Chemistry.
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder. All rights reserved.
dc.titleBinding Immobilization of Phorbol Esters and Diacylglycerolsen_US

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  • Chemistry Senior Individualized Projects [860]
    This collection includes Senior Individualized Projects (SIP's) completed in the Chemistry Department. Abstracts are generally available to the public, but PDF files are available only to current Kalamazoo College students, faculty, and staff.

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