Restriction Mapping and Sequence Analysis for Plasmids Containing the Genes that Code for Bacterial Luciferase from Vibrio Fisheri
Krueger, Joanna Katherine
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Bioluminescence is the phenomenon of light emission from a living organism. All known bioluminescent systems result from oxidative enzyme catalyzed chemiluminescent reactions. The living organism described in this report is the bacterium, Vibrio fisheri ATCC (American Type Culture Collection) strain 7744. The enzyme may be viewed as a mixed-function oxygenase, or more specifically a flavin monooxygenase. Knowing which amino acid residues or regions of the enzyme that are most conserved among the luciferases of various species of bioluminescent organisms will shed light on the regions directly involved with the catalyzed reaction. The goals of my project were to map the DNA which codes for the luciferase protein in the bacterium Vibrio fisheri with several restriction enzymes and to prepare plasmids for use in sequencing the luciferase.If you are not a current K College student, faculty, or staff member, email email@example.com to request access to this SIP.