Ascorbic Acid and ATP Regulate Norepinephrine Biosynthesis in Isolated Chromaffin Vesicles from Bovine Adrenal Medulla
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Authors
Hartzell, William
Issue Date
1986
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
This question of the mechanism of ascorbic acid
enhancement of DBH has led us to study of ATP's role in
norepinephrine biosynthesis. Adenine tri-phosphate (ATP) is
known to be the universal power source for the body due to its
high energy phosphate bond. In the chomaffin granule it has been
shown to be involved in the transport of catecholamines. In
particular, ATP is required to transport dopamine from the
cytosol into the chromaffin granule for subsequent hydroxylation
by Dopamine beta-hydroxylase. Yet, no experiments have been
performed testing the function of ATP for norepinephrine
biosynthesis independent of transport. By using high
concentration of external dopamine we thought it would be possible to transport dopamine into chromaffin granules by diffussion, without the requirement of MgATP transport. If dopamine penetrates the granule membrane, then it will be
possible to test the presence and absence of MgATP on
norepinephrine biosynthesis directly.
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Description
iv, 39 p.
Citation
Publisher
Kalamazoo College
License
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