Ascorbic Acid and ATP Regulate Norepinephrine Biosynthesis in Isolated Chromaffin Vesicles from Bovine Adrenal Medulla
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This question of the mechanism of ascorbic acid enhancement of DBH has led us to study of ATP's role in norepinephrine biosynthesis. Adenine tri-phosphate (ATP) is known to be the universal power source for the body due to its high energy phosphate bond. In the chomaffin granule it has been shown to be involved in the transport of catecholamines. In particular, ATP is required to transport dopamine from the cytosol into the chromaffin granule for subsequent hydroxylation by Dopamine beta-hydroxylase. Yet, no experiments have been performed testing the function of ATP for norepinephrine biosynthesis independent of transport. By using high concentration of external dopamine we thought it would be possible to transport dopamine into chromaffin granules by diffussion, without the requirement of MgATP transport. If dopamine penetrates the granule membrane, then it will be possible to test the presence and absence of MgATP on norepinephrine biosynthesis directly.If you are not a current K College student, faculty, or staff member, email firstname.lastname@example.org to request access to this SIP.