Positive Interference with the Jaffe' Method for Determination of Creatinine
Poe, Brent T.
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A main clinical test given to subjects to survey the renal function is creatinine determination. Plasma and urinary concentrations of creatinine give a good estimate of the body's Glomerular Filtration Rate (GFR). GFR is a very important indicator of either proper or improper function of the body's renal system. Determination of creatinine is measured by reaction with alkaline picrate to produce a chromogen, but this reaction is not specific. Several other compounds found both in urine and plasma also react with picrate the result of which is a positive interference with the Jaffe' method for determination of creatinine. Examples of interfering compounds include glucose, ascorbate, acetoacetate, oxaloacetate, -ketoglutarate, and pyruvate. The Jaffe' method is the most commonly used means of creatinine determination since creatinine is endogenously produced in the human body and its determination can be performed with inexpensive, stable reagents and a photometer.If you are not a current K College student, faculty, or staff member, email email@example.com to request access to this SIP.