Quantitative Determination of Monosaccharides by Microbore HPLC: A Glycoprotein Characterization Study
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Authors
Armstrong, Lisa J.
Issue Date
1986
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
In this study we were seeking a sensitive method to
quantitatively analyze the constituent monosaccharides of
glycoproteins by high pressure liquid chromatography.
Separation and quantification by gas-liquid chromatography
of tri-methylsilyl derivatives of the methyl glycosides is
presently the most common method of monosaccharide analysis. The instability of these volatile derivatives, as well as the lower detection limit by UV absorbance, make GC analysis less than optimal. However, because neutral and
amino sugars, as well as sialic acid can all be determined
by this one system, it has an advantage over many
available HPLC methods. We reviewed several of
methods and rejected them for this reason.
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Description
ii, 27 p.
Citation
Publisher
Kalamazoo College
License
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