Quantitative Determination of Monosaccharides by Microbore HPLC: A Glycoprotein Characterization Study
Armstrong, Lisa J.
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In this study we were seeking a sensitive method to quantitatively analyze the constituent monosaccharides of glycoproteins by high pressure liquid chromatography. Separation and quantification by gas-liquid chromatography of tri-methylsilyl derivatives of the methyl glycosides is presently the most common method of monosaccharide analysis. The instability of these volatile derivatives, as well as the lower detection limit by UV absorbance, make GC analysis less than optimal. However, because neutral and amino sugars, as well as sialic acid can all be determined by this one system, it has an advantage over many available HPLC methods. We reviewed several of methods and rejected them for this reason.If you are not a current K College student, faculty, or staff member, email email@example.com to request access to this SIP.