Quantitative Determination of Monosaccharides by Microbore HPLC: A Glycoprotein Characterization Study

Loading...
Thumbnail Image
Authors
Armstrong, Lisa J.
Issue Date
1986
Type
Thesis
Language
en_US
Keywords
Research Projects
Organizational Units
Journal Issue
Alternative Title
Abstract
In this study we were seeking a sensitive method to quantitatively analyze the constituent monosaccharides of glycoproteins by high pressure liquid chromatography. Separation and quantification by gas-liquid chromatography of tri-methylsilyl derivatives of the methyl glycosides is presently the most common method of monosaccharide analysis. The instability of these volatile derivatives, as well as the lower detection limit by UV absorbance, make GC analysis less than optimal. However, because neutral and amino sugars, as well as sialic acid can all be determined by this one system, it has an advantage over many available HPLC methods. We reviewed several of methods and rejected them for this reason.
If you are not a current K College student, faculty, or staff member, email dspace@kzoo.edu to request access to this SIP.
Description
ii, 27 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder. All rights reserved.
Journal
Volume
Issue
PubMed ID
DOI
ISSN
EISSN